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| | Articles of Volume : 2 Issue : 8 ( 4, September - 2014) | | | | 112 | Extraction of carotenoid and thin layer chromatography (TLC), GC-MS, FT-IR and HPLC analysis of pharmaceutically important pigment astaxanthin from a new strain of Haematococcus pluvialis | | By : S. Elumalai , B. Infant Santhose and G. Rajesh Kanna | | Abstract : Astaxanthin is an important pharmaceutical compound obtained from
different sources of micro algae. But Haematococcus pluvialis alone plays a
significant role in high astaxanthin accumulation comparing with other
sources. Even though astaxanthin extraction from Haematococcus pluvialis is
plausible, since more number of new strains of Haematococcus pluvialis still
needs effective study on astaxanthin accumulation. This study reports on
astaxanthin accumulation, extraction and its analysis from a new strain of
Haematococcus pluvialis. Optimization for complete isolation of carotenoids
involves various extraction methods, and astaxanthin has been given great
importance. When compared to all the methods of extraction, treatment with
HCl banks high amount of astaxanthin due to the consistent corrosion of cell
wall. The results from Thin layer chromatography especially on astaxanthin
and its mono- and di-esters were similar in comparison with other studies from
the same organism of different strain. Polyunsaturated fatty acid and
saturated fatty acid occupies high percentage quantitatively with low amount
of monounsaturated fatty acids comparatively from GC-MS analysis. The
HPLC result coincides apparently with the HPLC chromatogram of the
previous studies of astaxanthin from Haematococcus pluvialis. | | | | Author Profile | Full-Text PDF |View Video | |
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